Coregistration Demonstrations

Coregistration - the alignment of two separate modalities, such as T1-weighted and T2-weighted images - is an important precursor to normalization. This is because 1) It aligns both the anatomical and functional images into the same space and orientation; and 2) Because any warps applied to the anatomical image can then be accurately applied to the functional images as well. You can create a homemade demonstration of this yourself, using nothing more than a deck of playing cards, a lemon, and a belt.



However, before doing either coregistration or normalization, often it is useful to manually set the coordinates of the anatomical image (or whichever image you will be warping to a standardized space) so that it is in as close an alignment with the template image as possible. Since the origins of both MNI and Talairach standardized spaces are located approximately at the anterior commissure, the origin of the anatomical image should be placed there as well; this provides a better starting point for the normalization process, and increases the likelihood of success. The following tutorial shows you how to do this, as well as what the anterior commissure looks like.



Once this is done, you are ready to proceed with the coregistration step. Usually the average EPI image - output from the realignment step - will be used as the source image, while the anatomical image will be used as the reference image (the image that is moved around). Then, these warps are applied to the functional images to bring everything into harmonious alignment.